Heavy Metal Removal Investigation in Conventional Activated Sludge Systems

Magdi Buaisha, Saziye Balku, Şeniz Özalp Yaman


The combination of industrial and domestic wastewater in municipal WWTPs (waste water treatment plants) may be economically profitable, but it increases the difficulty of treatment, and also has some detrimental effects on the biomass and causes a low-quality final effluent. The present study evaluates the treatment process both in the presence and absence of heavy metals using ASM3 (activated sludge model no.3) so as to improve the model by means of incorporating other novel inhibitory kinetic and settler models. The results reveal that the presence of heavy metal, a case study for copper and cadmium at a concentration of 0.7 mgL−1 in a biological treatment system has a negative effect on heterotrophic bacteria concentration by 25.00 %, and 8.76 % respectively. Meanwhile, there are no important changes in COD (chemical oxygen demand), SS (total suspended solids) and TN (total nitrogen) in the final effluent in the conventional system. However, all these parameters are acceptable and consistent with EU Commission Directives. The results indicate that ASM3 can predict and provide an opportunity of the operation for an activated sludge wastewater treatment plant that receives the effluent from an industrial plant.


Activated Sludge; ASM3; Heavy Metal; Heterotrophs; Kinetic Models.


Fu, Fenglian, and Qi Wang. “Removal of Heavy Metal Ions from Wastewaters: A Review.” Journal of Environmental Management 92, no. 3 (March 2011): 407–418. doi:10.1016/j.jenvman.2010.11.011.

Ong, Soon-An, Eiichi Toorisaka, Makoto Hirata, and Tadashi Hano. ScienceAsia 36, no. 3 (2010): 204. doi:10.2306/scienceasia1513-1874.2010.36.204.

Ma, Yukun, Prasanna Egodawatta, James McGree, An Liu, and Ashantha Goonetilleke. “Human Health Risk Assessment of Heavy Metals in Urban Stormwater.” Science of The Total Environment 557–558 (July 2016): 764–772. doi:10.1016/j.scitotenv.2016.03.067.

Zhang, Lei, Yuexian Zeng, and Zhengjun Cheng. “Removal of Heavy Metal Ions Using Chitosan and Modified Chitosan: A Review.” Journal of Molecular Liquids 214 (February 2016): 175–191. doi:10.1016/j.molliq.2015.12.013.

Çeçen, Ferhan, Neslihan Semerci, and Ayşe Gül Geyik. “Inhibition of Respiration and Distribution of Cd, Pb, Hg, Ag and Cr Species in a Nitrifying Sludge.” Journal of Hazardous Materials 178, no. 1–3 (June 15, 2010): 619–627. doi:10.1016/j.jhazmat.2010.01.130.

Principi, P., F. Villa, M. Bernasconi, and E. Zanardini. “Metal Toxicity in Municipal Wastewater Activated Sludge Investigated by Multivariate Analysis and in Situ Hybridization.” Water Research 40, no. 1 (January 2006): 99–106. doi:10.1016/j.watres.2005.10.028.

Cabrero, Alberto, Sara Fernandez, Fernando Mirada, and Julian Garcia. “Effects of Copper and Zinc on the Activated Sludge Bacteria Growth Kinetics.” Water Research 32, no. 5 (March 1998): 1355–1362. doi:10.1016/s0043-1354(97)00366-7.

Dilek, Filiz B., Celal F. Gokcay, and Ulku Yetis. “Combined Effects of Ni(II) and Cr(VI) on Activated Sludge.” Water Research 32, no. 2 (February 1998): 303–312. doi:10.1016/s0043-1354(97)00225-x.

Pamukoglu, M. Yunus, and Fikret Kargi. “Copper(II) Ion Toxicity in Activated Sludge Processes as Function of Operating Parameters.” Enzyme and Microbial Technology 40, no. 5 (April 2007): 1228–1233. doi:10.1016/j.enzmictec.2006.09.005.

Pamukoglu, M. Yunus, and Fikret Kargi. “Mathematical Modeling of copper(II) Ion Inhibition on COD Removal in an Activated Sludge Unit.” Journal of Hazardous Materials 146, no. 1–2 (July 2007): 372–377. doi:10.1016/j.jhazmat.2006.12.033.

Sun, Fu-Lin, Lei-Lei Fan, and Guang-Jian Xie. “Effect of Copper on the Performance and Bacterial Communities of Activated Sludge Using Illumina MiSeq Platforms.” Chemosphere 156 (August 2016): 212–219. doi:10.1016/j.chemosphere.2016.04.117.

Wu, Xiaohui, Yang Yang, Gaoming Wu, Juan Mao, and Tao Zhou. “Simulation and Optimization of a Coking Wastewater Biological Treatment Process by Activated Sludge Models (ASM).” Journal of Environmental Management 165 (January 2016): 235–242. doi:10.1016/j.jenvman.2015.09.041.

Man, Yi, Wenhao Shen, Xiaoquan Chen, Zhou Long, and Marie-Noëlle Pons. “Modeling and Simulation of the Industrial Sequencing Batch Reactor Wastewater Treatment Process for Cleaner Production in Pulp and Paper Mills.” Journal of Cleaner Production 167 (November 2017): 643–652. doi:10.1016/j.jclepro.2017.08.236.

Pai, T.Y., S.C. Wang, H.M. Lo, C.F. Chiang, M.H. Liu, R.J. Chiou, W.Y. Chen, P.S. Hung, W.C. Liao, and H.G. Leu. “Novel Modeling Concept for Evaluating the Effects of Cadmium and Copper on Heterotrophic Growth and Lysis Rates in Activated Sludge Process.” Journal of Hazardous Materials 166, no. 1 (July 2009): 200–206. doi:10.1016/j.jhazmat.2008.11.009.

Juliastuti, S.R., J. Baeyens, C. Creemers, D. Bixio, and E. Lodewyckx. “The Inhibitory Effects of Heavy Metals and Organic Compounds on the Net Maximum Specific Growth Rate of the Autotrophic Biomass in Activated Sludge.” Journal of Hazardous Materials 100, no. 1–3 (June 2003): 271–283. doi:10.1016/s0304-3894(03)00116-x.

Henze, M., W. Gujer, T. Mino, and M. van Loosedrecht. “Activated Sludge Models ASM1, ASM2, ASM2d and ASM3.” Water Intelligence Online 5, no. 0 (December 30, 2015). doi:10.2166/9781780402369.

Takács, Imre, Gilles G. Patry, and Daniel Nolasco. “A Dynamic Model of the Clarification-Thickening Process.” Water Research 25, no. 10 (October 1991): 1263–1271. doi:10.1016/0043-1354(91)90066-y.

Tchobanoglous, G., Burton, F.L. “Wastewater Engineering: Treatment, Disposal and Reuse,” third ed. McGraw-Hill, New York, (1991): 1334.

Balku, Saziye. “Comparison Between Alternating Aerobic–anoxic and Conventional Activated Sludge Systems.” Water Research 41, no. 10 (May 2007): 2220–2228. doi:10.1016/j.watres.2007.01.046.

Directive, EU Urban Wastewater. "Council Directive of 21. May 1991 concerning urban waste water treatment (91/271/EEC)." J. Eur. Commun 34 (1991): 40.

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DOI: 10.28991/cej-2020-03091484


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